Active Hemicellulose Compound (AHCC) Enhances
NK Cell Activity of Aged Mice in Vivo
M. Ghoneum, Y. Ninomiya, M. Torabi, G. Gill and
A. Wojani. C.
Drew University of Medicine and Science, Department of Otolaryngology,
and Dermatology, 1621 East 120th Street, Los Angeles, CA 90059.
Daiwa Pharmaceutical Co. Ltd. 1-16-19 Sangenjaya Setagayaku, Tokyo.
Adjuvant Nutrition in Cancer Treatment Sponsored by: American
College of Nutrition & Cancer Treatment Centers of America.
(Continuing Education activity for 16 hours in CATEGORY I of the
Physicians Recognition Award of the AMA.)
Active Hemicellulose Compound (AHCC) is hemicellulose which originated
from rice and is biologically modified using carbohydrase separated
from Lentinus Edodes to increase its immunomodulatory function.
In the present investigation, we evaluated the ability of AHCC
to stimulate in vivo NK cytotoxic reactivity. Old C57BL/6 mice
were injected i.p. daily at a concentration of 30 mg/kg/day. At
2, 5 and 14 days at post treatment, peritoneal erudite and apleen
were examined for tissue cellularity and NK activity using 4-hr.
Cr-release assay against YAC-1 tumor cells. The results demonstrate
that; 1) AHCC generated peritoneal cytotoxic cells having the
characteristics of NK cells with high levels of granularity. The
induction was observed as early as 2 days, (750-900% of control)
and maintained at high levels with continuous injections, 2) Peritoneal
macrophage did not exhibit antitumor activity nor act as accessory
cells for NK cells, 3) No significant induction of splenic NK
cell activity, and 4) AHCC treated mice induced a significant
increase in peritoneal (300-500%) and splenic cellularity (150-190%),
suggesting that AHCC acts a mitogen factor in vivo. AHCC could
be considered as a potent biological response modifier and its
anti-cancer activity may be through post NK immunomodulation.